Cell Culture:Article Title: Menopausal Status Associated With Docetaxel-Induced Vascular Dysfunction in Breast Cancer Patients.
Article Snippet: .. Human microvascular endothelial cells (ATCC CRL-3243) were cultured in Medium MCDB131 (Thermo Fisher Scientific) supplemented with 10 ng/mL epidermal growth factor (Thermo Fisher Scientific), 1 μg/mL hydrocortisone (Merck), 10 mmol/L glutamine (Thermo Fisher Scientific), and 10% Fetal bovine serum (Thermo Fisher Scientific) with the addition of a 1% mixture of penicillin and streptomycin (Thermo Fisher Scientific). .. After an initial 4 hours of withdrawal of growth supplements from the culture, cells were incubated with 100 nmol/L docetaxel or 100 nmol/L docetaxel and 100 nmol/L 17β-estradiol (Merck) or vehicle (solvent) for 24 hours at 37 ◦ C in a humidified atmosphere with 5% CO 2 .
Article Title: Hallmarks of Sublethal Endothelial Injury Are Differentially Induced by Cuminum cyminum Extracts with Distinct Phytochemical Profiles.
Article Snippet: .. Human microvascular endothelial cells (HMEC-1) were cultured with GibcoTM DMEM (Dulbecco’s modified Eagle’s medium) medium, 10% fetal bovine serum, 1% Anti-Anti GibcoTM (10,000 μg/mL streptomycin, 25 μg/mL amphotericin B and 10,000 units/mL penicillin), 10 mM glutamine, 1 μg/mL hydrocortisone and 10 ng/mL EGF (endothelial growth factor) based on ATCC® guidelines. .. Cells were cultured in a humidified environment with 5% CO2 at 37 ◦C in Thermofisher® incubator (ThermoFisher Scientific, Waltham, MA, USA).
Article Title: A comparison of sequential density ultracentrifugation and density gradient ultracentrifugation for the proteomic analysis of high-density lipoprotein
Article Snippet: .. Briefly, human microvascular endothelial cells (HMEC-1, ATCC-CRL-3243, LGC Standards, Middlesex, UK) were cultured according to manufacturer recommendations. .. HMEC-1 were preincubated with HDL (based on 300 μg/mL apoA-I) for four hours before the addition of 5 ng/mL TNF α for 24 h. Cells were lysed in radioimmunoprecipitation assay buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1% v/v Triton X-100, 0.5% m/v sodium deoxycholate, 0.1% m/v sodium dodecyl sulphate, 1 mM ethylenediaminetetraacetic acid, 10 mM NaF, pH 7.4, supplemented with 1 × Roche Complete mini protease inhibitor cocktail tablet (Sigma-Aldrich #11836153001)) and western blotting performed to detect vascular cell adhesion molecule 1 (VCAM-1), using a mouse anti-human VCAM-1 antibody (Santa Cruz Biotechnology, sc-13160) and donkey anti-mouse IgG IRDye 800cw antibody (LI-COR, # 926-32212).
Article Title: Hallmarks of Sublethal Endothelial Injury Are Differentially Induced by Cuminum cyminum Extracts with Distinct Phytochemical Profiles
Article Snippet: .. Human microvascular endothelial cells (HMEC-1) were cultured with GibcoTM DMEM (Dulbecco’s modified Eagle’s medium) medium, 10% fetal bovine serum, 1% Anti-Anti GibcoTM (10,000 μg/mL streptomycin, 25 μg/mL amphotericin B and 10,000 units/mL penicillin), 10 mM glutamine, 1 μg/mL hydrocortisone and 10 ng/mL EGF (endothelial growth factor) based on ATCC ® guidelines. .. Cells were cultured in a humidified environment with 5% CO 2 at 37 °C in Thermofisher ® incubator (ThermoFisher Scientific, Waltham, MA, USA).
Article Title: hucMSCs alleviate LPS-induced endothelial glycocalyx damage by secreting Cxcl12 to activate CXCR4.
Article Snippet: .. Cell culture hucMSCs were sourced from Shandong Qilu Stem Cell Engineering Co., Ltd, and human microvascular endothelial cells (HULEC-5a) were purchased from the American Type Culture Collection (ATCC) . ..
Modification:Article Title: Hallmarks of Sublethal Endothelial Injury Are Differentially Induced by Cuminum cyminum Extracts with Distinct Phytochemical Profiles.
Article Snippet: .. Human microvascular endothelial cells (HMEC-1) were cultured with GibcoTM DMEM (Dulbecco’s modified Eagle’s medium) medium, 10% fetal bovine serum, 1% Anti-Anti GibcoTM (10,000 μg/mL streptomycin, 25 μg/mL amphotericin B and 10,000 units/mL penicillin), 10 mM glutamine, 1 μg/mL hydrocortisone and 10 ng/mL EGF (endothelial growth factor) based on ATCC® guidelines. .. Cells were cultured in a humidified environment with 5% CO2 at 37 ◦C in Thermofisher® incubator (ThermoFisher Scientific, Waltham, MA, USA).
Article Title: Hallmarks of Sublethal Endothelial Injury Are Differentially Induced by Cuminum cyminum Extracts with Distinct Phytochemical Profiles
Article Snippet: .. Human microvascular endothelial cells (HMEC-1) were cultured with GibcoTM DMEM (Dulbecco’s modified Eagle’s medium) medium, 10% fetal bovine serum, 1% Anti-Anti GibcoTM (10,000 μg/mL streptomycin, 25 μg/mL amphotericin B and 10,000 units/mL penicillin), 10 mM glutamine, 1 μg/mL hydrocortisone and 10 ng/mL EGF (endothelial growth factor) based on ATCC ® guidelines. .. Cells were cultured in a humidified environment with 5% CO 2 at 37 °C in Thermofisher ® incubator (ThermoFisher Scientific, Waltham, MA, USA).
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